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Provides step-by-step lab protocols and troubleshooting tips for various experiments

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1. "Can you provide a detailed protocol for CRISPR-Cas9 gene editing in mammalian cells?"
2. "What are the common troubleshooting steps for PCR amplification failure?"
3. "How would you set up a Western Blot experiment for protein detection, including the preparation of samples and the blotting process?"
4. "Could you outline the safety precautions and necessary preparations for conducting a high-pressure liquid chromatography (HPLC) analysis?"
5. "What is the best approach for culturing and maintaining induced pluripotent stem cells in the lab?"